Место издания:Samarkand State University named after Sharof Rashidov Samarkand
Первая страница:33
Последняя страница:33
Номер статьи:O-22
Аннотация:Microplastics are increasingly detected in diverse food matrices, raising questions
regarding their reliable quantification [1–2]. Recent studies have reported the presence of
small-size microplastics in agricultural and livestock products, such as milk, honey, and
meat [3–5]. Among such products, chicken eggs represent a nutritionally valuable but
analytically challenging biological matrix, combining lipid-rich yolk and protein based
white, which complicates isolation and quantification of microplastics. Currently, there
are no studies on the methodology of sample preparation of chicken eggs for
microplastics. Thus, the aim of this study was to develop performance parameters for the
quantitative determination of microplastics in chicken eggs using fluorescence
microscopy as a reliable and sensitive method for small size microplastics.
Several digestion protocols were tested to achieve effective degradation of eggs while
preserving polymer particles. The most efficient protocol was found to be alkaline
hydrolysis at elevated temperature, followed by neutralization of the digested egg
solution. This procedure ensured minimal matrix interferences and high reproducibility
of subsequent microplastics detection.
For selective visualization, microplastics isolated from egg samples were stained with a
commercially available fluorescent dye, enabling reliable recognition of polymer
microparticles among other organic residues. The trueness of the proposed analytical
method was assessed through several spike and recovery experiments.
The developed approach was applied to eggs from several commercial producers. The
results demonstrated that the proposed method provides accurate and reproducible
quantification of microplastics in chicken eggs, presenting a valuable analytical tool for
monitoring microplastic contamination in food products.
