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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Mechanical properties soft hydrogels: assessment by scanning ion-conductance microscopy and atomic force microscopyстатья
- Авторы: Tikhonova Tatiana N., Efremov Yuri M., Kolmogorov Vasilii, Iakovlev Aleksei, Sysoev Nikolay, Timashev Peter S., Fadeev Victor V., Tivtikyan Alexander, Salikhov Sergey, Gorelkin Petr, Korchev Yuri, Erofeev Alexandr, Shirshin Evgeny
- Журнал: Soft Matter
- Год издания: 2024
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- DOI: 10.1039/d4sm00966e
- Аннотация: The growing interest in biomimetic hydrogels is due to their successful applications in tissue engineering, 3D cell culturing and drug delivery. Major characteristics of hydrogels include swelling, porosity, degradation rate, biocompatibility, and mechanical properties. Poor mechanical properties can be regarded as the main limitation for the use of hydrogels in tissue engineering, and advanced techniques for its precise evaluation are of interest. The current research aims to demonstrate the suitability of scanning ion conductance microscopy (SICM) for assessing the stiffness of various hydrogels – Fmoc-FF peptide hydrogel, acrylamide and gelatin, – which differ by two orders of magnitude in Young's modulus (E). We provide a direct comparison between SICM measurements and atomic force microscopy (AFM) data, the latter being a widely used method for assessing the mechanical properties of scaffolds. The results of these methods showed good agreement, however, for materials with various stiffness two SICM-based approaches – application of hydrostatic pressure and application of intrinsic force – should be used. For hydrogels with Young’s modulus more than 2.5 kPa the application SICM using hydrostatic pressure is recommended, whereas for soft materials with E ~ 200-400 Pa the technique using intrinsic colloidal pressure can be applied. We have shown that SICM and AFM methods can be used for the evaluation of soft hydrogel mechanical properties with nanometer resolution, while SICM is a completely non-invasive method, which requires minimum influence on a sample structure.
- Добавил в систему: Тихонова Татьяна Николаевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | экспертное_заключение.pdf | экспертное_заключение.pdf | 1,4 МБ | 25 февраля 2025 [KolmogorovVS] | |
| 2. | Полный текст | Soft_matter_2024.pdf | 1,7 МБ | 18 ноября 2024 [Tikhonovath] |