6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotypeстатья

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Дата последнего поиска статьи во внешних источниках: 22 февраля 2018 г.

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[1] 6s rna in rhodobacter sphaeroides: 6s rna and prna transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype / D. Elkina, L. Weber, M. Lechner et al. // RNA Biology. — 2017. — Vol. 14, no. 11. — P. 1627–1637. The function of 6S RNA, a global regulator of transcription, was studied in the photosynthetic alfa-proteobacterium Rhodobacter sphaeroides. The cellular levels of R. sphaeroides 6S RNA peak toward the transition to stationary phase and strongly decrease during extended stationary phase. The synthesis of so-called product RNA transcripts (mainly 12–16-mers) on 6S RNA as template by RNA polymerase was found to be highest in late exponential phase. Product RNA ≥ 13-mers are expected to trigger the dissociation of 6S RNA:RNA polymerase complexes. A 6S RNA deletion in R. sphaeroides had no impact on growth under various metabolic and oxidative stress conditions (with the possible exception of tert-butyl hydroperoxide stress). However, the 6S RNA knockout resulted in a robust growth defect under high salt stress (0.25 M NaCl). Remarkably, the sspA gene encoding the putative salt stress-induced membrane protein SspA and located immediately downstream of the 6S RNA (ssrS) gene on the antisense strand was expressed at elevated levels in the delta-ssrS strain when grown in the presence of 250 mM NaCl. [ DOI ]

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