Hyperglycemia exacerbates ischemic stroke not through increased generation of hydrogen peroxide

Kotova, D.A.; Ivanova, A.D.; Pochechuev, M.S.; Kelmanson, I.V.; Khramova, Y.V.; Tiaglik, A.; Sudoplatov, M.A.; Trifonova, A.P.; Fedotova, A.; Morozova, K.; Katrukha, V.A.; Sergeeva, A.D.; Raevskii, R.I.; Pestriakova, M.P.; Solotenkov, M.A.; Stepanov, E.A.; Tsopina, A.S.; Moshchenko, A.A.; Shestopalova, M.; Zalygin, A.; Fedotov, I.V.; Fedotov, A.B.; Oleinikov, V.; Belousov, V.V.; Semyanov, A.; Brazhe, N.; Zheltikov, A.M.; Bilan, D.S.

Авторы: Kotova Daria A., Ivanova Aleksandra D., Pochechuev Matvei S., Kelmanson Ilya V., Khramova Yulia V., Tiaglik Alisa, Sudoplatov Mark A., Trifonova Arina P., Fedotova Anna, Morozova Kseniia, Katrukha Veronika A., Sergeeva Anastasia D., Raevskii Roman I., Pestriakova Mariia P., Solotenkov Maxim A., Stepanov Evgeny A., Tsopina Aleksandra S., Moshchenko Aleksandr A., Shestopalova Milena, Zalygin Anton, Fedotov Ilya V., Fedotov Andrei B., Oleinikov Vladimir, Belousov Vsevolod V., Semyanov Alexey, Brazhe Nadezda, Zheltikov Aleksei M., Bilan Dmitry S.
Журнал: Free Radical Biology and Medicine
Том: 208
Год издания: 2023
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 153
Последняя страница: 164
DOI: 10.1016/j.freeradbiomed.2023.08.004
Аннотация: Diabetes is one of the significant risk factors for ischemic stroke. Hyperglycemia exacerbates the pathogenesis of stroke, leading to more extensive cerebral damage and, as a result, to more severe consequences. However, the mechanism whereby the hyperglycemic status in diabetes affects biochemical processes during the development of ischemic injury is still not fully understood. In the present work, we record for the first time the real-time dynamics of H2O2 in the matrix of neuronal mitochondria in vitro in culture and in vivo in the brain tissues of rats during development of ischemic stroke under conditions of hyperglycemia and normal glucose levels. To accomplish this, we used a highly sensitive HyPer7 biosensor and a fiber-optic interface technology. We demonstrated that a high glycemic status does not affect the generation of H2O2 in the tissues of the ischemic core, while significantly exacerbating the consequences of pathogenesis. For the first time using Raman microspectroscopy approach, we have shown how a sharp increase in the blood glucose level increases the relative amount of reduced cytochromes in the mitochondrial electron transport chain in neurons under normal conditions in awake mice.
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