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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Circulating Plasma MicroRNA in Patients With Active Acromegalyстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Lutsenko A., Belaya Z., Nikitin A., Solodovnikov A., Lapshina A., Koshkin P., Vorontsova M., Rozhinskaya L., Melnichenko G., Dedov I.
- Журнал: Journal of Clinical Endocrinology and Metabolism
- Том: 107
- Номер: 2
- Год издания: 2022
- Издательство: The Endocrine Society
- Местоположение издательства: United States
- Первая страница: 500
- Последняя страница: 511
- DOI: 10.1210/clinem/dgab695
- Аннотация: Context. Excessive production of growth hormone causes marked multiorgan changes in patients with acromegaly, which may involve epigenetic mechanisms. Objective. To evaluate differences in circulating microRNAs (miRNAs) associated with chronic growth hormone overproduction in adults. Design and Setting. A cross-sectional case-control study was conducted at a tertiary medical center. Participants. We enrolled 12 consecutive patients with acromegaly along with 12 age- and sex-matched controls in the discovery phase of the study and then extended this cohort to 47 patients with acromegaly and 28 healthy controls for the validation study. Main Outcome Measures. Plasma miRNAs were quantified by next-generation sequencing (NGS) in the discovery phase. Levels of selected miRNAs were validated on extended cohorts using reverse transcription quantitative polymerase chain reaction (RT-qPCR), compared between groups, and correlated with clinical parameters. Results.Based on NGS data, we selected 3 plasma miRNAs downregulated in patients with acromegaly compared to healthy controls: miR-4446-3p −1.317 (P = 0.001), miR-215-5p −3.040 (P = 0.005), and miR-342-5p −1.875 (P = 0.013) without multiplicity correction for all 3 miRNAs. These results were confirmed by RT-qPCR in the validation phase for 2 miRNAs out of 3: miR-4446-3p (P < 0.001, Padjusted < 0.001), area under the receiver-operator curve (AUC) 0.862 (95% CI 0.723-0.936; P < 0.001) and miR-215-5p (P < 0.001, Padjusted < 0.001), AUC 0.829 (95% CI 0.698-0.907; P < 0.001) to differentiate patients with acromegaly from healthy controls. Conclusions. In a 2-phase experiment using 2 different techniques we found and validated the downregulation of plasma miR-4446-3p and miR-215-5p in patients with acromegaly compared to healthy subjects, which makes them promising biomarkers for further research.
- Добавил в систему: Воронцова Мария Владимировна