Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Gafarova, E.R.; Grebenik, E.A.; Lazhko, A.E.; Frolova, A.A.; Kuryanova, A.S.; Kurkov, A.V.; Bazhanov, I.A.; Kapomba, B.S.; Kosheleva, N.V.; Novikov, I.A.; Shekhter, A.B.; Golubeva, E.N.; Soloviova, A.B.; Timashev, P.S.

Авторы: Gafarova Elvira R., Grebenik Ekaterina A., Lazhko Alexey E., Frolova Anastasia A., Kuryanova Anastasia S., Kurkov Alexandr V., Bazhanov Ilya A., Kapomba Byron S., Kosheleva Nastasia V., Novikov Ivan A., Shekhter Anatoly B., Golubeva Elena N., Soloviova Anna B., Timashev Peter S.
Журнал: Molecules
Номер: 25
Год издания: 2020
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 3923
DOI: 10.3390/molecules25173923
Аннотация: One of the leading trends in the modern tissue engineering is the development of neweffective methods of decellularization aimed at the removal of cellular components from a donortissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assistedprocessing has been proposed to improve the outcome of decellularization, reduce contaminationand time costs. The resulting products can serve as personalized tools for tissue-engineering therapyof various somatic pathologies. However, the decellularization of heterogeneous 3D structures, suchas the aortic root, requires optimization of the parameters, including preconditioning mediumcomposition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor,etc. In our work, using an ovine aortic root model, we performed a comparative analysis of theeffectiveness of decellularization approaches based on various combinations of these parameters.The protocols were based on the combinations of treatments in alkaline, ethanol or detergentsolutions with scCO2-assisted processing at different modes. Histological analysis demonstratedfavorable effects of the preconditioning in a detergent solution. Following processing in scCO2medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimatetensile strength and Young’s modulus of the aortic valve leaflets, while the integrity of theextracellular matrix was preserved.
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Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularizationстатья

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