Evaluation of Supercritical CO2-Assisted Protocols in a Model of Ovine Aortic Root Decellularizationстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 ноября 2020 г.
Аннотация:One of the leading trends in the modern tissue engineering is the development of neweffective methods of decellularization aimed at the removal of cellular components from a donortissue, reducing its immunogenicity and the risk of rejection. Supercritical CO2 (scCO2)-assistedprocessing has been proposed to improve the outcome of decellularization, reduce contaminationand time costs. The resulting products can serve as personalized tools for tissue-engineering therapyof various somatic pathologies. However, the decellularization of heterogeneous 3D structures, suchas the aortic root, requires optimization of the parameters, including preconditioning mediumcomposition, the type of co-solvent, values of pressure and temperature inside the scCO2 reactor,etc. In our work, using an ovine aortic root model, we performed a comparative analysis of theeffectiveness of decellularization approaches based on various combinations of these parameters.The protocols were based on the combinations of treatments in alkaline, ethanol or detergentsolutions with scCO2-assisted processing at different modes. Histological analysis demonstratedfavorable effects of the preconditioning in a detergent solution. Following processing in scCO2medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimatetensile strength and Young’s modulus of the aortic valve leaflets, while the integrity of theextracellular matrix was preserved.