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Prolonged oxygen depletion in microwounded cells of Chara corallina detected with novel O2 nanosensorsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 мая 2020 г.
- Авторы: Anna Alova, Alexander Erofeev, Petr Gorelkin, Tatyana Bibikova, Yury Korchev, Alexander Majouga, Alexander Bulychev
- Журнал: Journal of Experimental Botany
- Том: 71
- Номер: 1
- Год издания: 2020
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 386
- Последняя страница: 398
- DOI: 10.1093/jxb/erz433
- Аннотация: Primary physicochemical steps in microwounding of plants were addressed using electrochemical nano- and microprobes, with the focus on the role of oxygen in the wounding responses of individual plant cells. The electrochemical measurements of oxygen content were performed with carbon-filled quartz micropipettes having platinum-coated tips (oxygen nanosensors). This novel platinum nanoelectrodes are beneficial for understanding cell oxygen metabolism and can be implemented to study the redox biochemistry and biology of cells, tissues and organisms. We show here that microinjury of Chara corallina internodal cell with a tip of a glass micropipette is associated with a drastic decrease in O2 concentration at the vicinity of the stimulation site. This decrease is reversible and lasts for up to 40 minutes. Membrane stretching, Ca2+ influx and cytoskeleton rearrangements were found to be essential for localized oxygen depletion induced by cell wall microwounding. Inhibition of electron transport in chloroplasts or mitochondria did not affect the magnitude or timing of the observed response. In contrast, the inhibition of NADPH oxidase activity caused significant reduction in the amplitude of the oxygen decrease. We suggest that the discovered creation of localized anoxic conditions in response to cell wall puncture might be mediated by NADPH oxidase.
- Добавил в систему: Бибикова Татьяна Николаевна