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1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assayстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 марта 2016 г.
- Авторы: Aparin Ilya O., Farzan Valentina M., Veselova Olga A., Chistov Alexey A., Podkolzin Alexander T., Ustinov Alexey V., Shipulin German A., Formanovsky Andrey A., Korshun Vladimir A., Zatsepin Timofei S.
- Журнал: The Analyst
- Том: 141
- Год издания: 2016
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 1331
- Последняя страница: 1338
- DOI: 10.1039/c5an01767j
- Аннотация: An alkyl azide derivative of 1-phenylethynylpyrene (PEPy) dye was prepared and used in functionalization of oligonucleotides via click chemistry. Spectral and photophysical properties of PEPy-modified oligonucleotides as a single strand and in perfect or mismatched duplexes have been studied. A series of PEPy–Dabcyl fluorogenic TaqMan probes were synthesized and tested in qPCR. PEPy proved to be a superior substitute for AMCA as a short wavelength fluorescent dye for qPCR probes. PEPy probes were shown to reduce Cq (a fractional PCR cycle used for quantification) vs AMCA labeled probes thus improving the reliability of the detection. Moreover, a larger increase of fluorescence during amplification was observed in case of PEPy probes that makes this dye well suitable for end-point PCR technique. The study broadens the panel of fluorescent dyes suitable for the use in quantitative real-time PCR probes.
- Добавил в систему: Зацепин Тимофей Сергеевич