ИСТИНА

Karachi(Cliff News)Faculty of Science, Dadabhoy Institute of Higher Education has organized a Post 12th INTERNATIONAL BIENNIAL CONFERENCE (TIBC-PSM) 2019 Seminar on “Novel antibiotic SkQ1 targets bacterial bioenergetics and prevents biofilm formation” at Main Campus, DIHE.Guest Speaker was Dr. Pavel A. Nazatov who is ASM Young Ambassador to Russia and a Senior Research Scientist- Belozersky Institute Moscow State University. Dr. Pavel described about SkQ1, which is decyl-triphenyl phosphonium conjugate with plastoquinone, produced severe antibacterial effect on other Gram-positive bacteria, Mycobacterium sp. and Staphylococcus aureus, at submicromolar and micromolar concentrations, as determined by 2-fold broth microdilution and visualized by plots showing changes in optical density (OD) at 620 nm over time. He mentioned that SkQ1 exhibited much lower antibiotic activity towards Escherichia coli obviously due to the presence of the highly effective multidrug resistance pump AcrAB-TolC. Micromolar concentrations of SkQ1 caused a decrease in the membrane potential of B. subtilis in the minute time scale, whereas higher concentrations of SkQ1 caused a rapid drop of membrane potential to the level observed with the channel-forming antibiotic gramicidin A. SkQ1 increased fluorescence of ethidium bromide but did not cause an increase in fluorescence of the cell membrane-impermeant probe propidium iodide, therefore the action of SkQ1 on the membrane potential of B. subtilis was not due to a detergent effect on bacterial membrane.After the vote of thanks Rector Of DIHE Prof. Dr. Muhammad Mukhtar, Dean Faculty of Science Prof. Dr. Shahana Urooj Kazmi and all faculty members presented a memento to our honorable guest speaker.