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Chapter 2. In: A Comprehensive Guide to Chemiluminescence, Emitter Structure in the Firefly Luciferin-Luciferase System: A Historical Perspective.Pages 35-58книга
- Авторы: Ugarova N.N., Lomakina G.Yu
- Год издания: 2019
- Место издания: Nova Science Publishers, Inc United States
- Объём: 357 страниц
- ISBN: 978-1-53616-170-0
- Монография
- Аннотация: Bioluminescence of firefly luciferases is characterized by complex changes of the emission spectra and bioluminescence λmax occurring with changing pH, temperature, and the enzyme structure. Analysis of the literature data and the result of our own research on the nature of emitter in the firefly luciferin-luciferase system allows concluding that keto-enol tautomerization of the excited oxyluciferin molecule provides the most adequate explanation of the observed complex spectral changes. Only one emitter molecule can be present in the active center of the luciferase molecule, hence, emitter can be considered as an intramolecular probe reporting on the properties of its microenvironment in the enzyme active center. Superposition of two or three forms of the emitter recorded in the bioluminescence spectra implies that equilibrium of different conformations of the enzyme exist in the reaction medium with each generating its own form of the emitter.
- Добавил в систему: Угарова Наталья Николаевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Глава 2 в монографии | A_Comprehensive_Guide_to_Chemiluminescence_Chapter_2.pdf | 17,7 МБ | 2 декабря 2019 [UgarovaNN] |