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CONSTRUCTION OF PLANT EXPRESSION VECTOR OF GOGID GENE AND TRANSFORMATION OF MEDICAGO SATIVA Lстатья
- Авторы: Li Ju, Wen H.Yu, Gao H.W., Dzyubenko N., Chapurin V., Wang X.M.
- Журнал: ACTA AGRESTICA SINICA
- Том: 23
- Номер: 1
- Год издания: 2015
- Первая страница: 167
- Последняя страница: 172
- DOI: 10.11733/j.issn.1007-0435.2015.01.026
- Аннотация: Based on the Galega orientalis Gibberellin Receptor gene (GoGID) sequence, a cDNA fragment was cloned. By the aid of the pBI121 vector, the plant expression vector pBI121-GoGID was constructed using digestion and ligation method. The recombinant vector was verified by enzyme digestion. The plasmid was then transferred to Agrobacterium tumefaciens GV3101 by CaCl2 freeze-thaw method. Transgenic plants were obtained through somatic embryogenesis from leaves as explants by A.tumefaciens-mediated transformation. The nptⅡ and GUS gene existed in plasmid were tested by PCR in the genome of transgenic plants and all had positive results, indicating that the target gene has bean transferred into plant. The transgenic plants were also tested by Southern-blot and RT-PCR. This study will pave the path for further study of the function of GoGID in alfalfa biomass improvement.
- Добавил в систему: Дзюбенко Николай Иванович