LC-MS determination of steroidal glycosides from Dioscorea deltoidea Wall cell suspension culture: Optimization of pre-LC-MS procedure parameters by latin square designстатья

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Дата последнего поиска статьи во внешних источниках: 28 июня 2018 г.

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[1] Lc-ms determination of steroidal glycosides from dioscorea deltoidea wall cell suspension culture: Optimization of pre-lc-ms procedure parameters by latin square design / S. Boris, F. Elizaveta, S. Oleg et al. // Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. — 2018. — Vol. 1080. — P. 64–70. In this paper, the ultrasound assisted extraction method for isolation of steroidal glycosides from D. deltoidea plant cell suspension culture with a subsequent HPLC-MS determination was developed. After the organic solvent was selected via a two-factor experiment the optimization of extraction parameters was carried out using Latin Square 4 × 4 experimental design for the following parameters: extraction time, organic solvent concentration in extraction solution and the ratio of solvent to sample. It was also shown that the ultrasound-assisted extraction method is not suitable for isolation of steroidal glycosides from the D. deltoidea plant material. The results were confirmed using the multiple successive extraction method and refluxing extraction. Optimal conditions for the extraction of steroidal glycosides by the ultrasound-assisted extraction method were: extraction time, 60 min; acetonitrile (water) concentration in extraction solution, 50% (50%); the ratio of solvent to sample, 400 mL/g. Also, the developed method was tested on D. deltoidea cell suspension cultures of different terms and conditions of cultivation. The completeness of the extraction was confirmed using the multiple successive extraction method. [ DOI ]

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