Аннотация:We found new Kdn-TULA in the cell walls of various actinomycetes (Streptomyces, Arthrobacter, Brevibacterium, Promicromonospora), which differ in morphology and habitat. Their common structural feature is a backbone composed of residues of α-Kdnp and a hexose or GlcNAc. The following structures of the new Kdn-TULA were established:
•→6)-β-D-Galp-(1→9)-[RO-4]-α-Kdnp-(2→, where R = H, CH3 or α-D-GlcpNAc
(S. coelicolor M145)
•→3)-β-D-Galp-(1→9)-α-Kdnp-(2→ (S. albus J1074, R1-100)
•→6)-β-D-Glcp-(1→8)-α-Kdnp-(2→ (B. aurantiacum VKM Ac-2111Т,
A. protophormiae VKM Ac-2104T, S. albus VKM Ac-35T, S. coelicolor Ac-738T)
•→6)-β-D-GlcpNAc-(1→8)-α-Kdnp-(2→ (Arthrobacter sp. VKM Ac-2550, Ac-2549)
•6)-[RO-3]-α-D-Glcp-(1→4)-[Pyr-7,9]-α-Kdnp-(2→, where R = H or α-D-Galp,
Pyr indicates pyruvic acid acetal (P. citrea VKM Aс-665T)
In the cell walls of all actinomycetes studied, the Kdn-TULA are accompanied by various other glycopolymers, viz. teichoic and teichuronic acids, poly(glycosyl 1-phosphates), and neutral polysaccharides.
Being polyanionic polymers, the Kdn-TULA can bind cations, impart negative charge to the cell surface, control the autolysin activity, and are involved in the cell communication within the microbial community and the environment, including higher organisms.