New superparamagnetic fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 nanoparticles for biological applicationsстатья

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[1] New superparamagnetic fluorescent fe@c-c5on2h10-alexa fluor 647 nanoparticles for biological applications / A. S. Garanina, I. I. Kireev, I. B. Alieva et al. // Наносистемы: физика, химия, математика. — 2018. — Vol. 9, no. 1. — P. 1–3. The structure and physical properties of superparamagnetic Fe@C nanoparticles (Fe@C NPs) as well as their uptake by living cells and behavior inside the cell were investigated. Magnetic capacity of Fe@C NPs was compared with Fe7C3@C NPs investigated in our previous work, and showed higher value of magnetic saturation, 75 emu.g−1(75 Am 2·kg−1), against 54emu.g−1(54 Am 2·kg−1) for Fe7C3@C. The surface of Fe@C NPs was alkylcarboxylated and further aminated for covalent linking to the molecules of fluorochrome Alexa Fluor 647. Fluorescent Fe@C-C5ON2H10-Alexa Fluor 647 NPs (Fe@C-Alexa NPs) were incubated with HT1080 human fibrosarcoma cells and investigated using fluorescent, confocal laser scanning and transmission electron microscopy. No toxic effect on the cell physiology was observed. In a magnetic field, the NPs became aligned along the magnetic lines inside the cells.

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