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Microplate chemiluminescent assay for HBV DNA detection using 3-(10′- phenothiazinyl)propionic acid/N-morpholinopyridine pair as enhancer of HRP-catalyzed chemiluminescenceстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 марта 2018 г.
- Авторы: Bodulev O.L., Gribas A.V., Sakharov I.Yu
- Журнал: Analytical Biochemistry
- Том: 543
- Год издания: 2018
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 33
- Последняя страница: 36
- DOI: 10.1016/j.ab.2017.11.026
- Аннотация: A sensitive sandwich assay for hepatitis B virus (HBV) DNA detection based on use of commercial CL-ELISA microplates was developed. To reveal the target the covalent conjugate of reporter oligonucleotide and horseradish peroxidase (HRP) was synthesized. An employment of enhanced chemiluminescence reaction, where 3-(10′-phenothiazinyl)propionic acid/N-morpholinopyridine pair was used as enhancer of HRP-catalyzed chemiluminescence, permitted to measure the enzyme activity of the conjugate with high sensitivity. Under the favorable conditions the limit of detection and a linear range of the assay were 3 pM and 0.07–2.0 nM, respectively. The coefficient of variation (CV) for determination of HBV DNA concentrations within the working range was lower than 4%. The obtained results demonstrated that the developed assay had high sensitivity and precision.
- Добавил в систему: Сахаров Иван Юрьевич