Development of fluorescence polarisation immunoassay for carbofuran in food and environmental water samplesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 апреля 2015 г.
Авторы:
Jin-Yi Yang,
Yan Zhang,
Hong Wang,
Zhen-Lin Xu,
Eremin Sergei A.,
Yu-Dong Shen,
Qing Wu,
Hong-Tao Lei,
Yuan-Ming Sun
Аннотация:A simple and rapid method for determination of carbofuran was developed using the fluorescence polarization immunoassay (FPIA). Three tracers with different lengths of bridge (0, 2 and 6-carbon bridge) between the hapten molecule 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy)carbonyl]-amino]-butanoic acid (BFNB) and 5-aminofluorescein (AF), fluoresceinthiocarbamyl ethylenediamine (EDF), fluoresceinthiocarbamyl hexylenediamine (HDF), were synthesized and their binding response with anti-carbofuran specific antibody were evaluated. The physicochemical parameters were optimized for the FPIA. The AF labeled BFNB conjugate (BFNB-AF) was found to be the optimal tracer for FPIA of carbofuran. The detection limit of carbofuran, IC50 value and the working range were 2.3, 48.8 and 7.4202.2 μg/L, respectively; and the reaction time was only 10 min. The average recovery from spiked water and vegetable samples was 86.995.4% and the mean coefficient of variation (CV) was 6.2% for inter-assay and 8.7% for intra-assay, which showed good reproducibility for FPIA. Thus, the developed FPIA method exhibited the potential for the rapid and accurate determination of carbofuran in agricultural and environmental samples.