Optimization and comparison of different techniques for complete extraction of saponins from T. terrestrisстатья

Информация о цитировании статьи получена из Scopus, Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2018 г.

Работа с статьей


[1] Optimization and comparison of different techniques for complete extraction of saponins from t. terrestris / B. Sarvin, E. Stekolshchikova, I. Rodin et al. // JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS. — 2018. — Vol. 8. — P. 75–82. Ultrasound-assisted (UAE), refluxing (RE), low pressure refluxing (LPRE) and Soxhlet (SE) extraction techniques were developed for determination of steroidal saponins content in Tribulus terrestris L. Extraction parameters for UAE (solvent base: methanol, ethanol, isopropanol and acetonitrile; the ratio of solvent to soil: 50–400 mL/g; isopropanol and acetonitrile concentration: 30–100%; extraction time: 15–120 min), RE (solvent base: isopropanol and acetonitrile; the ratio of solvent to soil: 50–400 mL/g; isopropanol concentration: 30–80%) and SE (extraction time: 4, 8, 12, 18 and 24 h) methods were optimized by single-factor-experiment. Thermal decomposition of protodioscin was observed after 60 min of RE and 12 h of SE. Extraction time and temperature were optimized via double-factor-experiment for most promising RE and LPRE techniques. Comparison of UAE with RE, LPRE and SE methods was made for protodioscin and dioscin which are major polar and less polar T. terrestris saponins, correspondingly. The equal maximum of extraction yield was achieved using RE, LPRE and SE in optimized extraction conditions. Also, the possibility of complete isolation of target compounds at the first extraction step was shown in multi-step extraction experiment for RE and LPRE methods and reached saponin yields were also achieved after 12 h of SE. The accuracy for all studied extraction techniques was confirmed by spiking of the plant material with standards before extraction and HPLC-ESI–MS analysis. [ DOI ]

Публикация в формате сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл скрыть