Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome Cстатья

Информация о цитировании статьи получена из Scopus, Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 апреля 2015 г.

Работа с статьей

Прикрепленные файлы


Имя Описание Имя файла Размер Добавлен
1. Shilov_et_al_Cytochrome_c_2014.pdf Shilov_et_al_Cytochrome_c_2014.pdf 1,5 МБ 23 мая 2016 [shilov_evgeny]

[1] Mouse lymphomyeloid cells can function with significantly decreased expression levels of cytochrome c / E. Shilov, I. Kislyakov, E. Gorshkova et al. // Biochemistry (Moscow). — 2014. — Vol. 79, no. 12. — P. 1412–1422. Cytochrome c is an indispensable electron carrier in the mitochondrial respiratory chain and also an important mediator of the internal pathway triggering apoptosis. Mice with a complete deficiency of the Cycs gene encoding the somatic cytochrome c die during the embryogenesis. Using the technology of LoxP-cre-dependent tissue-specific recombination, we obtained some mouse strains with significantly reduced expression of cytochrome c in certain cell types ("conditional genetic knockdown"). This knockdown was achieved by abrogation of the normal splicing of the Cycs locus pre-mRNA due to an additional acceptor site inside the stop-cassette neo(r). Previously, we observed embryonic lethality in homozygous mice with the same knockdown of cytochrome c in all cells of the organism. In the present work we studied two novel mouse strains with conditional knockdown of the Cycs gene in T lymphocytes and macrophages. Somewhat surprisingly, the mice of these two strains under normal conditions were not phenotypically different from the wild-type mice, either on the whole organism level or on the level of activity of individual target cells. Thus, the amount of cytochrome c in lymphomyeloid cells does not affect their development and normal functioning. [ DOI ]

Публикация в формате сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл сохранить в файл скрыть