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An enhanced chemiluminescence resonance energy transfer system based on target recycling G-guadruplexes/hemin DNAzyme catalysis and its application in ultrasensitive detection of DNAстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 июня 2015 г.
- Авторы: Jia Chen, Yong Huang, Vdovenko Marina M., Sakharov Ivan Yu, Guifa Su, Shulin Zhao
- Журнал: Talanta
- Том: 138
- Год издания: 2015
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 59
- Последняя страница: 63
- DOI: 10.1016/j.talanta.2015.02.004
- Аннотация: An enhanced chemiluminescence resonance energy transfer(CRET)system based on target recycling G-guadruplexes/hemin DNAzyme catalysis was developed for ultrasensitive detection of DNA. CRET system consists of luminol as chemiluminescent donor,and fluorescein isothiocyanate(FITC)as acceptor. The sensitive detection was achieved by using the system consisted of G-richedDNA, blockerDNA,and the Nb.BbvCI biocatalyst.Upon addition of target DNA to the system,target DNA hybridizes with the quasi-circular DNA structure,and forms a DNAduplex.The formation of DNA duplex triggers selective enzymatic cleavage of quasi-circular DNA by Nb.BbvCI, resulting in the release of target DNA and two G-riched DNAzyme segments. Released target DNA then hybridizes with another quasi-circularDNA structure to initiate the cleavage of the quasi-circular DNA structure.Eventually,each target DNA can go through many cycles,resulting in the digestion of many quasi-circular DNA structures,generating many G-riched DNAzyme segments. G-riched DNAzyme segment products assemble with hemin to form stable hemin/G-quadruplexes that exhibit peroxidase-like activity which can catalyze the oxidation of luminol by H2O2 to produce CL signals.In the presence of FITC, CL of luminol can excite FITC molecules,and thus produced CRET between thel uminol and FITC.This unique analysis strategy gives a detection limit down to 80fM,which is at least four orders of magnitude lower than that of unamplified DNA detection methods.
- Добавил в систему: Сахаров Иван Юрьевич