Noninvasive penetration of 5 nm hyaluronic acid molecules across the epidermal barrier (in vitro) and its interaction with human skin cellsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
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Дата последнего поиска статьи во внешних источниках: 10 февраля 2018 г.
Аннотация:Objective: Hyaluronic acid represents one of the major components of the extracellular
environment. The main challenge remains in the ability to deliver these molecules
noninvasively across the skin barrier, which can be overcome by the reduction in size
to an extent that allows these molecules to pass across the skin barrier.
The aim of this study was to measure the penetration and bioavailability of low molecular
weight hyaluronic acid to cross an epidermal barrier model.
Methods: Determining the quantity of hyaluronic acid in the test solutions was carried
with method of photocolorimetry analysis. Investigation of the interaction of cells with
LMWHA was studied with a confocal microscope.
Results: The study showed that LMWHA is able to cross the epidermis. Most effective
penetration level is during the first 6 hours reaching 75%, and then the concentration
started to decline and reached the equilibrium state within the following 2 hours.
Confocal laser microscopy demonstrated different distribution and behavior of these
molecules among the keratinocytes and fibroblasts.
Conclusion: Reducing the size of hyaluronic acid to 5 nm enhance their transport
across the epidermal layer. The concentration of hyaluronic acid molecules was higher
on the fibroblast surface in comparison to their extracellular environment.