A SIMPLE METHOD FOR DETERMINING PROTEIN-BOUND HOMOCYSTEINE AND CYSTEINE IN HUMAN PLASMA BY CAPILLARY ELECTROPHORESISстатья Исследовательская статья

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Дата последнего поиска статьи во внешних источниках: 24 апреля 2018 г.

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[1] A simple method for determining protein-bound homocysteine and cysteine in human plasma by capillary electrophoresis / E. D. Virus, A. V. Ivanov, M. I. Kuchukova et al. // Biotecnologia Aplicada. — 2016. — Vol. 33, no. 4. — P. 4301–4304. A capillary electrophoresis method with UV detection has been developed for the determination of protein-bound cysteine and homocysteine in human plasma based on the combination of derivatization step with pH-mediated base stacking. Centrifugal ultrafiltration was carried out to clarify plasma proteins from salts and low-molecular weight compounds. Thereafter, the sample was incubated with dithiothreitol to reduce the disulfides and release protein-bound aminothiols. The released thiols were derivatized with thiocarbonyldiimidazole and injected into the capillary electrophoresis by voltage. Due to the stacking effects it is possible to perform a considerable on-line pre-concentration of the analytes. The proposed approach allows to reach a detection limit of 1 μmol/L in blood plasma using 48.5 cm total length 50-μm i.d. uncoated capillary. The coefficient of variation of the assay was within ± 5 % for both homocysteine and cysteine.

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