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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Fluorescent microscopy approaches of quantitative soil microbial analysisтезисы доклада
- Автор: and Lubov Polyanskaya Konstantin Ivanov
- Сборник: European Geosciences Union General Assembly 2015 [USB flash drive]
- Серия: Geophysical Research Abstracts
- Том: 17
- Тезисы
- Год издания: 2015
- Издательство: Copernicus GmbH on behalf of the European Geosciences Union
- Местоположение издательства: Germany
- Аннотация: Classical fluorescent microscopy method was used during the last decades in various microbiological studies of terrestrial ecosystems. The method provides representative results and simple application which is allow to use it both as routine part of amplitudinous research and in small-scaled laboratories. Furthermore, depending on research targets a lot of modifications of fluorescent microscopy method were established. Combination and comparison of several approaches is an opportunity of quantitative estimation of microbial community in soil. The first analytical part of the study was dedicated to soil bacterial density estimation by fluorescent microscopy in dynamic of several 30-days experiments. The purpose of research was estimation of changes in soil bacterial community on the different soil horizons under aerobic and anaerobic conditions with adding nutrients in two experimental sets: cellulose and chitin. Was modified the nalidixic acid method for inhibition of DNA division of gram-negative bacteria, and the method provides the quantification of this bacterial group by fluorescent microscopy. Established approach allowed to estimate 3-4 times more cells of gram-negative bacteria in soi
- Добавил в систему: Полянская Любовь Максимовна