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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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The dynamics of ATRX and the modified histones H3K9me3 and H4K5ac in the nucleus of mouse embryos during zygote genome activationтезисы доклада
- Авторы: Sailau Z., Bogolyubova I.
- Сборник: 25th Wilhelm Bernhard Workshop on the Cell Nucleus, Nizhny Novgorod, 19-22 June 2017. Programme and abstract book
- Тезисы
- Год издания: 2017
- Первая страница: 103
- Последняя страница: 103
- Аннотация: The changes of chromatin functional activity are known to occur during early mouse development, but mostly these have been studied in zygotes, when major epigenetic rearrangements take place. In elder embryos, nuclear distribution of the proteins involved in chromatin reorganization remains poorly studied. We used an immunolabeling approach to localize the chromatin-remodeling protein ATRX, tri-methylated histone H3 (H3K9me3) and acetylated histone H4 (H4K5ac) in the nuclei of mouse early embryos that are characterized by different levels of transcriptional activity. In early zygotes, a diffuse ATRX distribution prevails. During zygote genome activation (ZGA), ATRX tends to concentrate in local zones associated with a heterochromatin rim around the nucleolus precursor bodies (NPBs). Furthermore, additional ATRX-positive zones, not associated with NPBs, appear in the nucleoplasm of 2-cell embryos. In morulae, after the completion of ZGA, ATRX distribution becomes diffuse again. In the early zygote, before the onset of ZGA, a weak diffuse anti-H3K9me3 staining was detected in both female and male pronuclei. In late 2-cell stage, after the completion of ZGA, multiple H3K9me3-positive areas were revealed on the periphery of NPBs. Besides, H4K5ac-positive regions were clearly seen in early zygotes and 2-cell embryos, although H4K5ac is an epigenetic mark of active chromatin. These regions were predominantly located in association with the heterochromatin rim around the NPBs. H4K5ac was also detected in blocks of the peripheral heterochromatin. These clusters were more evident in early zygotes. ATRX was found to colocalize with the marks of either the repressed (H3K9me3) or the active (H4K5ac) chromatin in the transcriptionally active embryo nuclei but not in zygotic pronuclei prior to the ZGA. The colocalization zones were predominant on the periphery of some NPBs. At the same time, a few heterochromatic regions that contain ATRX but not the studied epigenetic markers, including H3K9me3 which is known as a functional partner of ATRX, were also found. In closing, the pictures of nuclear distribution of the studied proteins in mouse embryos during ZGA look rather unusual as compared with typical somatic cells. Our data confirm a unique functional status of the perinuclear heterochromatin during mammalian early development.
- Добавил в систему: Боголюбова Ирина Олеговна