Effects of the superparamagnetic iron oxide nanoparticles on the synthesis of the extracellular matrix proteins and their reorganization by the mesenchymal stem cellsстатья Тезисы

Статья опубликована в высокорейтинговом журнале

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Дата последнего поиска статьи во внешних источниках: 29 марта 2018 г.

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[1] Effects of the superparamagnetic iron oxide nanoparticles on the synthesis of the extracellular matrix proteins and their reorganization by the mesenchymal stem cells / N. M. Yudintceva, Y. A. Nashchekina, M. I. Blinova et al. // FEBS Journal. — 2017. — Vol. 284, no. supp 1. — P. 382–383. Therapy by mesenchymal stem cells (MSCs) has been reported to provide beneficial effects at different disorders treatment. Visualization of cells and indication of their role at tissue repair the superparamagnetic iron oxide nanoparticles (SPIONs) can be used. SPIONs can effectively and quickly label most cells in vitro, and date suggests that such labeling does not compromise the proliferation or differentiation of cells. However it is still unknown how the inclusion of SPIONs can influence the functional activity of cells. In this study, we analyzed the possibility of any SPIONs negative influence on cell function, in particular on synthesis of extracellular matrix (ECM) proteins and ECM reorganization by matrix metalloproteinases (MMPs) at 3D cell culture conditions. Materials and methods: Human MSCs were incubated with SPIONs (Fe concentration of 150 lg/ml) for one day in a CO2- incubator. 3D-scaffold based on poly(e-caprolactone) was used for cells cultivation. The cultivation of MSCs without SPIONs under 2D conditions was used as control. Cells viability and proliferation were assessed by Trypan blue and MTT assays. The evaluation of ECM proteins synthesis and ECM reorganization by MMPs was performed at the various cultivation terms by of protein electrophoresis, immunoblotting and zymography methods. The samples of mediums and scaffolds were analyzed separately. Results and conclusion: Essential distinctions of ECM proteins and MMPs synthesis for cells, which were containing and not containing SPIONs have been shown. The cells with SPIONs synthesized much more ECM proteins (collagen I and III types, fibronectin etc) and demonstrated higher MMPs activity then control cells. Thus, there was not any negative influence of SPIONs on cell functions. Opposite the SPIONs increased the ECM proteins synthesis and ECM reorganization by MMPs. [ DOI ]

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