Effects of the superparamagnetic iron oxide nanoparticles on the synthesis of the extracellular matrix proteins and their reorganization by the mesenchymal stem cellsстатьяТезисы
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 марта 2018 г.
Аннотация:Therapy by mesenchymal stem cells (MSCs) has been reported to
provide beneficial effects at different disorders treatment. Visualization
of cells and indication of their role at tissue repair the
superparamagnetic iron oxide nanoparticles (SPIONs) can be
used. SPIONs can effectively and quickly label most cells in vitro,
and date suggests that such labeling does not compromise the
proliferation or differentiation of cells. However it is still
unknown how the inclusion of SPIONs can influence the functional
activity of cells. In this study, we analyzed the possibility
of any SPIONs negative influence on cell function, in particular
on synthesis of extracellular matrix (ECM) proteins and ECM
reorganization by matrix metalloproteinases (MMPs) at 3D cell
culture conditions.
Materials and methods: Human MSCs were incubated with
SPIONs (Fe concentration of 150 lg/ml) for one day in a CO2-
incubator. 3D-scaffold based on poly(e-caprolactone) was used
for cells cultivation. The cultivation of MSCs without SPIONs
under 2D conditions was used as control. Cells viability and proliferation
were assessed by Trypan blue and MTT assays. The
evaluation of ECM proteins synthesis and ECM reorganization
by MMPs was performed at the various cultivation terms by of
protein electrophoresis, immunoblotting and zymography methods.
The samples of mediums and scaffolds were analyzed separately.
Results and conclusion: Essential distinctions of ECM proteins
and MMPs synthesis for cells, which were containing and not
containing SPIONs have been shown. The cells with SPIONs
synthesized much more ECM proteins (collagen I and III types,
fibronectin etc) and demonstrated higher MMPs activity then
control cells. Thus, there was not any negative influence of
SPIONs on cell functions. Opposite the SPIONs increased the
ECM proteins synthesis and ECM reorganization by MMPs.