|
ИСТИНА |
Войти в систему Регистрация |
Интеллектуальная Система Тематического Исследования НАукометрических данных |
||
Encapsulation of enzymes in silica nanocapsules formed by an amphiphilic precursor polymer in waterстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 ноября 2017 г.
- Авторы: Zhang Chi, Yan Kelu, Chunyan Hu, Zhao Yongliang, Chen Zhi, Zhu Xiaomin, Moeller Martin
- Журнал: Journal of Materials Chemistry B
- Том: 3
- Номер: 7
- Год издания: 2015
- Первая страница: 1261
- Последняя страница: 1267
- DOI: 10.1039/c4tb01701c
- Аннотация: In this work we report a fully aqueous and highly efficient method for the encapsulation of enzymes in silica nanocapsules during their formation process. In this approach, enzymes are first enclosed into unilamellar vesicles self-assembled by an amphiphilic precursor polymer - poly(ethylene glycol) substituted hyperbranched polyethoxysiloxane (PEG-PEOS) - in water. After subsequent condensation under basic conditions enzyme-loaded silica nanocapsules are obtained. Due to the significant volume shrinkage during the PEG-PEOS conversion, the encapsulation efficiency is very high, i.e. by adding only 5 wt% PEG-PEOS almost 50% of the enzyme from the solution is encapsulated. As compared with the free enzyme, the protease encapsulated by this means preserves almost 40% of its activity, exhibits significantly enhanced stability against the change of environmental conditions, and can be repeatedly regenerated without a significant activity loss.
- Добавил в систему: Чжу Сяоминь