Аннотация:Humic substance (HS) is a heterogeneous supramolecular ensemble of natural
organic compounds formed by oxidative decomposition of biomacromolecules in soils,
coals, waters, etc. It has a broad spectrum of biological activity, such as anti-inflammatory,
antibacterial, and antiviral. Inhibitory effect of HS on reproduction of a wide range of DNA
and RNA viruses reveals a possibility to apply HS against human pathogens with no
approved therapeutics. However, the exact structural composition of HS can not be
determined by modern analytical techniques and structure and the nature of HS bioactivity
remains unknown, thus complicating the process of development HS-based drugs. In this
study we investigated inhibitory effect of ten HS samples, obtained by standard protocols,
on enteroviruses and flaviviruses in cell-based assays and developed a technique for an
active component identification using high-resolution mass-spectrometry spectral data
analysis and chemical database search.