Homogeneous Chemiluminescent Determination of Mercury(II) using a Peroxidase-Mimicking DNAzyme Assayстатья

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Дата последнего поиска статьи во внешних источниках: 2 мая 2018 г.

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[1] Gribas A. V., Sakharov I. Y. Homogeneous chemiluminescent determination of mercury(ii) using a peroxidase-mimicking dnazyme assay // Analytical Letters. — 2018. — Vol. 51, no. 9. — P. 1280–1290. Environmental pollution in manufacturing sectors is often accompanied by the release of diverse forms of pollutants including heavy metals. Mercury is one of the most toxic heavy metals. Here, we describe a homogeneous chemiluminescent method for Hg2+ detection based on allosteric activation of peroxidase-mimicking DNAzyme and formation of Hg2+-thymine bonds in DNA duplex with T–T mismatches in the presence of mercury. The formation of such duplex increased the activity of peroxidase-mimicking DNAzyme. The analysis conditions and structures of probes were optimized. Under the favorable conditions the limit of detection and a linear range of the assay were 12 nM and 12-600 nM, respectively. The values of coefficient of variation measured within the working range varied from 0.7 to 3.0%. The study of cross-reactivity of Hg2+, Ag+, Pb2+, Ca2+, Zn2+, Bi3+, Ni2+, Co2+, Ba2+, Mn2+, Cd2+, Mg2+ and Cr3+ showed that only mercury in concentration nanoscale activates peroxidase-mimicking DNAzyme that indicates to high specificity of the developed Hg2+ assay. Thus, easy to use, specific, rapid and sensitive method for Hg2+ detection was developed. [ DOI ]

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