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Binding of Glycyl-tRNA synthetase to Mengovirus RNA stimulates translationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 4 марта 2026 г.
- Авторы: Droß Fabian, Gödert Tim, Fuchshuber Selena A., Nachev Dimitar V., Andreev Dmitri E., Fricke Markus, Ritsch Muriel, Gerresheim Gesche K., Repp Simeon, Noe Yannic, Rossbach Oliver, Marz Manja, Barth Patrick, Goesmann Alexander, Linne Uwe, Weber Axel, Kracht Michael, Shatsky Ivan N., Niepmann Michael
- Журнал: Nucleic Acids Research
- Том: 54
- Номер: 1
- Год издания: 2026
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Номер статьи: gkaf1451
- DOI: 10.1093/nar/gkaf1451
- Аннотация: Picornaviruses are small viruses with a plus-strand RNA genome in which RNA secondary structures bind cellular proteins to support viral translation and replication. Here, we characterize tRNA anticodon stem-loop-like structures in the 5'- and 3' untranslated regions (UTRs) of the RNA of Mengovirus, a member of the Cardiovirus group in the Picornaviridae family. These RNA elements specifically bind cellular Glycyl-tRNA synthetase (GARS). Mutation of the conserved CCA motifs in the loops of these GARS binding elements (GBEs) impairs binding, as does deletion of the anticodon binding domain of GARS. Mutation of the 3'-UTR GBE reduces Mengovirus translation early after transfection, independent of viral polymerase activity. The 3'UTR GBE is a stronger GARS binding site, and in reporter RNAs with the Mengovirus 5'- and 3'-UTRs, the 3'UTR GBE strongly contributes to recruitment of translation factors and ribosomes, thereby stimulating translation. In contrast, the 5'UTR GBE is a weaker GARS binding site, but its mutation has a stronger effect on translation. Therefore, we hypothesize that a GARS dimer binds strongly to an "anchor" site in the 3'UTR with one monomer, while the other monomer interacts with the 5'UTR to stimulate recruitment of translation factors and ribosomes.
- Добавил в систему: Антохина Оксана Геннадьевна