The Product of Yersinia pseudotuberculosis mcc Operon Is a Peptide-cytidine Antibiotic Activated Inside Producing Cells by the TldD/E Proteaseстатья

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[1] The product of yersinia pseudotuberculosis mcc operon is a peptide-cytidine antibiotic activated inside producing cells by the tldd/e protease / D. Tsibulskaya, O. Mokina, A. Kulikovsky et al. // Journal of the American Chemical Society. — 2017. — Vol. 139, no. 45. — P. 16178–16187. Microcin C is a heptapeptide-adenylate antibiotic produced by some strains of Escherichia coli. Its peptide part is responsible for facilitated transport inside sensitive cells where it is proteolysed with release of a toxic warhead - a non-hydrolysable aspartamidyl-adenylate, which inhibits aspartyl-tRNA synthetase. Recently, a microcin C homolog from Bacillus amyloliquefaciens containing a longer peptide part modified with carboxymethyl-cytosine instead of adenosine was described but no biological activity of this compound was revealed. Here, we characterize modified peptide-cytidylate from Yersinia pseudotuberculosis. As reported for B. amyloliquefaciens homolog, the initially synthesized compound contains a long peptide that is biologically inactive. This compound is subjected to endoproteolytic processing inside producing cells by the evolutionary conserved TldD/E protease. As a result, an 11-aminoacid long peptide with C-terminal modified cytosine residue is produced. This compound is exported outside the producing cell and is bioactive, inhibiting sensitive cells in the same way as E. coli microcin CProteolytic processing inside producing cells is a novel strategy of peptide-nucleotide antibiotics biosynthesis that may help control production levels and avoid toxicity to the producer. [ DOI ]

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