Аннотация:RNA is a huge class of molecules that perform a variety of functions in a livingcell. Each type of RNA must undergo a specific sequence of maturation steps to function. In this work, we study one of the main stages of spliceosomal small nuclear RNAs(snRNAs) maturation. These molecules are synthesized as longer precursors, afterwhich the 3′ tails of snRNAs are gradually cut off, first in the nucleus and then inthecytoplasm. However, the exact length of snRNA precursors at each of these stages ispoorly understood. The Integrator protein plays crucial role in the nuclear maturationof snRNA. The aim of this study is to determine the 3 ′ -end maturation dynamics of varioussnRNAs. Using the method of isolating newly synthesized RNA via 5-Ethynyluridinefollowed by high-throughput sequencing, we suggested the precursor length of eachsnRNA and calculated the rate of 3′-end shortening. The effect of the knockdownofthe genes encoding the Integrator protein subunits on the snRNAmaturationdynamics was also investigated. snRNAs have many copies in the genome, only someof which serve as a template for the synthesis of functional molecules. By focusingonthe most expressed copies, we showed that 3′ region transformation dynamics differnot only between snRNA types, but also between different copies of the same type. Differences in the masturation of the 3′-ends of snRNAs expands the understandingofthe dynamic regulation of splicing.
