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Identification of Differential Genes by Suppression Subtractive Hybridization: II. Subtractive Hybridizationстатья
- Автор: Rebrikov D.V.
- Журнал: CSH protocols
- Том: 2008
- Год издания: 2008
- Издательство: Cold Spring Harbor Laboratory
- Местоположение издательства: Cold Spring Harbor, NY, United States
- Первая страница: pdb.prot4856
- Аннотация: INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representing genes that are either overexpressed or exclusively expressed in one population as compared to another. It can also be used for comparison of genomic DNA populations. This protocol describes a method for subtractive hybridization using adapter-ligated tester and RsaI-digested driver DNA samples.
- Добавил в систему: Ребриков Денис Владимирович