A novel source of dihomo-gamma-linolenic acid: possibilities and limitations of DGLA production in the high-density cultures of the ∆5 desaturase-mutant microalga Lobosphaera incisaстатья

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Дата последнего поиска статьи во внешних источниках: 10 февраля 2016 г.

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[1] A novel source of dihomo-gamma-linolenic acid: possibilities and limitations of dgla production in the high-density cultures of the ∆5 desaturase-mutant microalga lobosphaera incisa / S. Abu-Ghosh, D. Pal-Nath, D. Markovitch et al. // European Journal of Lipid Science and Technology. — 2014. — Vol. 117, no. 6. — P. 760–766. The Δ5 desaturase mutant (P127) of the microalga Lobosphaera incisa is a promising organism for large-scale production of the valuable LC-PUFA dihomo-gamma-linolenic acid (DGLA, 20:3 n-6). We examined the potential of P127 for DGLA production under nitrogen (N) starvation conditions, triggering the deposition of DGLA in triacylglycerols, and developed a strategy for optimization of the DGLA productivity in high-density cultures. Towards this end, the effects of initial biomass concentration (1, 2, and 4 g L−1) and PAR irradiance (170 and 400 μmol m-2 s-1) on DGLA and total fatty acid (TFA) production were studied. The highest DGLA and TFA percentages (10 and 38% of dry weight, respectively) were displayed by the cultures initiated at 1 g L−1 and grown under a moderate irradiance. Higher irradiances and lower starting biomass content facilitated oleic acid accumulation at the expense of DGLA. Maximum volumetric productivities of TFA and DGLA were recorded in the cultures started at 2 g L−1 biomass and grown under 400 μmol PAR m−2 s−1. We show that a sufficiently high starting culture density should be combined with a mild light stress to facilitate the production of biomass enriched in DGLA-containing triacylglycerols. [ DOI ]

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