Genotypic analysis of Russian isolates of Phytophthora infestans from the Moscow region, Siberia and Far Eastстатья

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Дата последнего поиска статьи во внешних источниках: 17 ноября 2014 г.

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[1] Genotypic analysis of russian isolates of phytophthora infestans from the moscow region, siberia and far east / S. Elansky, A. Smirnov, Y. Dyakov et al. // Journal of Phytopathology. — 2001. — Vol. 149, no. 10. — P. 605–611. Phytophthora infestans samples were collected during 1997and 1998 at multiple sites in Russia from Sakhalin Island in the Far East across Siberia (nine sites, 160 isolates) to the Moscow region (four sites, 325 isolates). Additionally, 12 isolates that were obtained previously were analyzed. All isolates were analyzed for mating type, and sensitivity to metalaxyl. Isolates from within any of the nine sites outside of the Moscow region were monomorphic for mating type and nearly monomorphic for metalaxyl resistance. In contrast, both A1 and A2 isolates were detected in the Moscow region, and these isolates were also polymorphic for metalaxyl resistance. In two sites in Siberia only A2 mating type strains were detected, in the other six sites in Siberia and in Sakhalin Island, only A1 mating types were detected. A subset of isolates (n=191) was also analyzed for pathotype. All isolates were highly complex (many compatibilities, with a mean value of compatibility of ca 8.4 (max = 10). All isolates (n = 43) from Sakhalin Island were compatible with all 10 of the R-genes tested. A further subset of isolates (n= 70, including 12 isolates collected before 1997) was analyzed for genotype at the Glucose-6-phosphate isomerase and Peptidase loci, mtDNA haplotypes, and RFLP pattern using the RG57 probe. The US-1 clonal lineage (previously dominant) was not detected in the 1997-1998 sample. The population of P. infestans near Moscow in 1997 and 1998 was highly diverse with 15 unique genotypes (including both mating types) among a sample of 18 isolates. In contrast the populations of P. infestans in Siberia had limited diversity, with only three multilocus genotypes detected and most populations were dominated by the SIB-1 clonal lineage. This lineage accounted for 31of the 39 strains collected in Siberia that were assayed for multilocus genotype.

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