Аннотация:Objective: This study aimed to examine the efficiency of newly formulated drugs based on mechanochemical modification of two widely used anthelmintic substancesin suspension formulationsagainst the main classes of helminths and parasitic arthropods.Materials and Methods: Solid-phase co-grinding of two substances, i.e., ivermectin (IM) andniclosamide (NS), with licorice extract and sodium succinate was performed in liquid-phase processing to obtain suspension formulations. Drug preparations were administered to the sheepof the experimental groups (5 experimental groups of 8 heads) with different concentrations ofactive substances, i.e., 3.0, 5.0, and 10.0 mg/kg of body weight (BW) NS and 0.2-mg/kg BW IM.Aqueous suspensions of the original substances in dosages of 0.2-mg/kg BW IM, 10.0-mg/kg NS,and placebo were included as controls. Efficacy of the formulations against gastrointestinal strongyles, Trichuris spp., Moniezia spp., and nasal bot larvae (Oestrus ovis) was assessed in naturallyinfected sheep by the number of surviving adult parasites post-treatment in the gastrointestinaltract, nasal passages, and sinuses.Results: Formulated suspensions showed an increased solubility of 5.5–13.1 times compared tothe original substances. Treatment of sheep with the SusIN-10 drug containing 0.2-mg/kg BW IMand 10.0-mg/kg NS showed 100% efficiency against gastrointestinal strongyles, Moniezia, andnasal bot larvae. Formulations containing 0.2-mg/kg BW IM and 3.0- and 0.5-mg/kg NS couldeliminate all of the Trichuris worms. Administration of original substances of IM and NS with recommended dosages did not result in the adult parasites’ complete elimination.Conclusion: Modification of the anthelmintic substances through mechanochemical methodsmade it possible to create formulations with a targeted spectrum of action, significantly higherwater solubility, and optimal parasiticidal activity.
