Genetically Controlled Iron Oxide Biomineralization in Encapsulin Nanocompartments for Magnetic Manipulation of a Mammalian Cell Line

Efremova, M.V.; Wiedwald, U.; Sigmund, F.; Bodea, S.; Ohldag, H.; Feggeler, T.; Meckenstock, R.; Panzl, L.N.; Francke, J.; Beer, I.; Ivleva, N.P.; Alieva, I.B.; Garanina, A.S.; Semkina, A.S.; Curdt, F.; Josten, N.; Wintz, S.; Farle, M.; Lavrijsen, R.; Abakumov, M.A.; Winklhofer, M.; Westmeyer, G.G.

Авторы: Efremova Maria V., Wiedwald Ulf, Sigmund Felix, Bodea Silviu-Vasile, Ohldag Hendrik, Feggeler Thomas, Meckenstock Ralf, Panzl Lorenz N., Francke Jeroen, Beer Irina, Ivleva Natalia P., Alieva Irina B., Garanina Anastasiia S., Semkina Alevtina S., Curdt Franziska, Josten Nicolas, Wintz Sebastian, Farle Michael, Lavrijsen Reinoud, Abakumov Maxim A., Winklhofer Michael, Westmeyer Gil G.
Журнал: Advanced Functional Materials
Том: 2418013
Год издания: 2025
Издательство: John Wiley & Sons Ltd.
Местоположение издательства: United Kingdom
Первая страница: 1
Последняя страница: 18
DOI: 10.1002/adfm.202418013
Аннотация: Magnetic nanoparticles have proven invaluable for biomechanicalinvestigations due to their ability to exert localized forces. However, cellulardelivery of exogenous magnetic agents often results in endosomal entrapment,thereby limiting their utility for manipulating subcellular structures. Thisstudy characterizes and exploits fully genetically controlled biomineralizationof iron-oxide cores inside encapsulin nanocompartments to enablemagnetic-activated cell sorting (MACS) and magnetic cell manipulation.The fraction of MACS-retained cells showed substantial overexpressionof encapsulins and exhibited both para- and ferrimagnetic responses withmagnetic moments of 10−15 A m2 per cell, comparable to standard exogenouslabels for MACS. Electron microscopy revealed that MACS-retained cellscontained densely packed agglomerates of ≈30 nm iron oxide cores consistingof ultrafine quasicrystalline ordered nuclei within an amorphous matrixof iron, oxygen, and phosphorus. Scanning transmission X-ray microscopy,X-ray absorption spectroscopy, and Raman microspectroscopy confirmed thatthe iron-oxide species are consistent with ferric oxide (Fe2O3). In addition,the encapsulin-overexpressing MACS-retained cells can be manipulated bya magnetic needle and regrown in patterns determined by magnetic gradients.This study demonstrates that the formation of quasicrystalline iron oxidewith mixed para/ferrimagnetic behavior in the cytosol of mammalian cellsenables magnetic manipulation without the delivery of exogenous agents.
Добавил в систему: Алиева Ирина Борисовна

	ИСТИНА	Войти в систему Регистрация
	Интеллектуальная Система Тематического Исследования НАукометрических данных
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

Интеллектуальная Система Тематического Исследования НАукометрических данных

Genetically Controlled Iron Oxide Biomineralization in Encapsulin Nanocompartments for Magnetic Manipulation of a Mammalian Cell Lineстатья

Прикрепленные файлы