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Stannous Chloride-Modified Glass Substrates for Biomolecule Immobilization: Development of Label-Free Interferometric Sensor Chips for Highly Sensitive Detection of Aflatoxin B1 in Cornстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Orlov Alexey V., Zolotova Maria O., Novichikhin Denis O., Belyakov Nikolai A., Protasova Svetlana G., Nikitin Petr I., Sinolits Artem V.
- Журнал: Biosensors
- Том: 14
- Номер: 11
- Год издания: 2024
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 531
- Последняя страница: 531
- DOI: 10.3390/bios14110531
- Аннотация: This study presents the development of stannous chloride (SnCl2)-modified glass substrates for biomolecule immobilization and their application in fabricating sensor chips for label-free interferometric biosensors. The glass modification process was optimized, identifying a 5% SnCl2 concentration, a 45 min reaction time, and a 150 °C drying temperature as conditions for efficient protein immobilization. Based on the SnCl2-modified glass substrates and label-free spectral-phase interferometry, a biosensor was developed for the detection of aflatoxin B1 (AFB1)—a highly toxic and carcinogenic contaminant in agricultural products. The biosensor realizes a competitive immunoassay of a remarkable detection limit as low as 26 pg/mL of AFB1, and a five-order dynamic range. The biosensor performance was validated using real corn flour samples contaminated with Aspergillus flavus. The proposed approach not only provides a powerful tool for AFB1 detection for food safety monitoring but also demonstrates the potential of SnCl2-modified substrates as a versatile platform for the development of next-generation biosensors.
- Добавил в систему: Синолиц Артём Вадимович