Pitfalls in optical clearing agents induced enhancement of blood microcirculation imaging

Lugovtsov, A.; Moldon, P.; Ermolinskiy, P.; Maksimov, M.; Timoshina, P.; Li Pengcheng; Priezzhev, A.

Авторы: Lugovtsov Andrei, Moldon Pavel, Ermolinskiy Petr, Maksimov Matvei, Timoshina Polina, Li Pengcheng, Priezzhev Alexander
Сборник: Book of abstracts of 17th International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2024)
Тезисы
Год издания: 2024
Место издания: Sanya, China
Первая страница: 54
Последняя страница: 54
Номер статьи: [CRI-6] PIBM2024-0823-1
Аннотация: The optical clearing agents (OCA) are used to improve the visualization of blood capillaries in the skin and blood microcirculation assessment with speckle contract imaging. Digital capillaroscopy (DC) is a method widely used for visualization of human capillaries in vivo, in particular, the nail bed capillaries. However, OCA being applied to the tissue surface can penetrate to the bloodstream. Most of OCA are osmotically active. They can change the microrheological properties of blood and locally alter the diagnosed blood flow, introducing errors in measurements using DC. The goal of this work was to demonstrate the possible effect of OCA blood microcirculation parameters as well as to find the ways to minimize such effect without giving up the optical clearing (OC). For this reason, we examined the efficiency of 15 different OCAs. The effect of OC was assessed using the OCT on the nail bed area of volunteers’ finger after the application of OCAs. The effect of OCAs on RBC aggregation and deformability properties in vitro was measured by diffuse light scattering method. We showed that these properties of RBC dramatically changed when blood was incubated with OCAs. We also showed that the most effective OCA for enhancing the quality of blood capillaries imaging by DC technique is glycerol. We demonstrated that the capillary blood flow velocities measured by capillaroscopy of the nail bed depend on the type of OCA used to visualize the capillaries. We hypothesize that the mechanism for the observed differences is most likely that the OCAs penetrate into the capillaries and change the properties of the vessel walls and RBC. The capabilities of OC to improve the visualization of capillary blood flow and necessity to correct the parameters measured using DC are discussed. This work was supported by the RSF grant No. 23-45-00027.
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