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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Fragment of mucin MUC1 extracellular domain enhances metastatic potential of cancer cells in vitroтезисы доклада
- Авторы: Syrkina M.S., Rubtsov M.A., Kondratenko J.D., Veiko V.P.
- Сборник: FEBS JOURNAL
- Серия: Special issue
- Том: 280
- Тезисы
- Год издания: 2013
- Место издания: Blackwell Publishing Inc United Kingdom
- Первая страница: 466
- Последняя страница: 466
- Аннотация: The speculations about the role of MUC1 which is known to be overexpressed in glandular cancers on metastatic potential of cancer cells originate from the data indicating that cell malignization correlates with the change of apical mucin MUC1 localization to peripheral [1]. But the role of MUC1 in cancer metastasizing is not clear yet [2-4]. The major problem is the absence of adequate cell models. The aim of this study was to create cell models presenting different fragments of human mucin MUC1 extracellular domain on cell surface. Genetic constructions were generated on the basis of plasmid vector pEGFP-N3. These constructions contain fusion genes coding chimeric proteins composed of different combinations of mucin MUC1 functional domains and identification markers – FLAGepitope located at the N-terminus and EGFP located at the C-terminus of the chimeric proteins. The constructions were used for stable transformation of human cancer cells HT-29. Transformants obtained were characterized by means of flow cytometry. Low expression level of endogenous mucin MUC1 and high expression level of the recombinant proteins were confirmed by real-time PCR. Microscopic examination of transformed cells confirmed localization of fusion proteins on its plasmatic membrane. Cells obtained are used to be a quite applicable model of MUC1- expressing cancers and might be used for study the role of different functional fragments of mucin MUC1 in metastasizing. Cells obtained were tested for the ability to penetrate and migrate through the endothelium by using an artificial system. It was shown that the expression of fragments of mucin MUC1 extracellular domain leads to an increase in the proportion of cells capable to overcome the endothelial barrier. Furthermore, a fragment has been identified, the presentation of which on the cell surface leads to the greatest increase in the proportion of cells migrated.
- Добавил в систему: Сыркина Марина Сергеевна