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Engineering Cas9: next generation of genomic editorsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Kovalev Maxim A., Davletshin Artem I., Karpov Dmitry S.
- Журнал: Applied Microbiology and Biotechnology
- Том: 108
- Номер: 209
- Год издания: 2024
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- DOI: 10.1007/s00253-024-13056-y
- Аннотация: The Cas9 endonuclease of the CRISPR/Cas type IIA system from Streptococcus pyogenes is the heart of genome editingtechnology that can be used to treat human genetic and viral diseases. Despite its large size and other drawbacks, S. pyogenesCas9 remains the most widely used genome editor. A vast amount of research is aimed at improving Cas9 as a promisinggenetic therapy. Strategies include directed evolution of the Cas9 protein, rational design, and domain swapping. The frstgeneration of Cas9 editors comes directly from the wild-type protein. The next generation is obtained by combining mutations from the frst-generation variants, adding new mutations to them, or refning mutations. This review summarizes anddiscusses recent advances and ways in the creation of next-generation genomic editors derived from S. pyogenes Cas9.
- Добавил в систему: Ковалев Максим Александрович