The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of theChlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)

Kazakov, G.A.; Zaitsev, P.A.; Chudaev, D.A.; Parshina, E.Y.; Moiseenko, A.V.; Zaitseva, A.A.; Fedorenko, T.A.; Bokov, M.G.; Mojzeš, P.; Lobakova, E.S.; Solovchenko, A.E.

Авторы: Kazakov Georgiy A., Zaitsev Petr A., Chudaev Dmitry A., Parshina Eugeniya Yu, Moiseenko Andrey V., Zaitseva Anna A., Fedorenko Tatiana A., Bokov Maksim G., Mojzeš Peter, Lobakova Elena S., Solovchenko Alexei E.
Журнал: Russian Journal of Plant Physiology
Год издания: 2024
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
DOI: 10.1134/S1021443724606098
Аннотация: The productivity of microalgal cultures and their resilience to unfavorable conditions is largelydetermined by the availability of mineral nutrients, particularly nitrogen. Nitrogen starvation is a strongstressor that induces a broad range of responses in microalgae at the cell and cell population (culture) levels.These responses such as lipogenesis and secondary carotenogenesis are widely used in biotechnology toobtain valuable secondary metabolites of microalgae. It was believed that microalgal cells lack specific structures that function as long-term nitrogen depot, but recent studies assigned this role to microcrystalline inclusions constituted by nitrogenous bases (most often guanine). It is also known that purine microcrystals arebiophotonic structures widespread in nature. These structures modify the intensity and spectral compositionof radiation in illuminated cells and tissues of living organisms. In this regard, we studied (i) the dynamics ofthe formation of guanine crystals in the cells of green carotenogenic microalgae from the genus Coelastrella(C. thermophila NAMSU CM1/23 and C. rubescens IPPAS C-2066) depending on the availability of nitrogenin the medium and (ii) the effect of the presence of these crystals on cell resilience to high light intensity.Nitrogen-rich crystal inclusions were accumulated in cells when the cultures reached the stationary growthphase in a medium with ample nitrate nitrogen. During rapid growth, these inclusions were hardly detectablein the cells and disappeared during cultivation in the absence of nitrogen. Optical polarization microscopyand Raman microspectroscopy demonstrated that these nitrogen-rich inclusions are birefringent microcrystals composed of guanine. C. thermophila cells harboring abundant guanine crystals showed resistance toshort-term (10–15 min) exposure to high-intensity light (600 μmol PAR quanta/m 2 s). The results obtainedare discussed in the context of the multifaceted role of guanine crystals in the stress tolerance of microalgae.
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The Turnover and Possible Physiological Significance of Purine Crystals in the Cells of theChlorophytes from the Genus Coelastrella (Scenedesmaceae, Chlorophyta)статья

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