Probing of nucleic acid compaction using low-frequency Raman spectroscopy

Sosorev, A.Y.; Parashchuk, O.D.; Chicherin, I.V.; Trubitsyn, A.A.; Trukhanov, V.A.; Baleva, M.V.; Piunova, U.E.; Kharlanov, O.G.; Kamenski, P.; Dmitry, Y.P.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 июля 2024 г.

Авторы: Sosorev Andrey Yu, Parashchuk Olga D., Chicherin Ivan V., Trubitsyn Artem A., Trukhanov Vasiliy A., Baleva Maria V., Piunova Ulyana E., Kharlanov Oleg G., Kamenski Piotr, Dmitry Yu Paraschuk
Журнал: Physical Chemistry Chemical Physics
Том: 26
Номер: 25
Год издания: 2024
Издательство: Royal Society of Chemistry
Местоположение издательства: United Kingdom
Первая страница: 17467
Последняя страница: 17475
DOI: 10.1039/d3cp05857c
Аннотация: Compaction of nucleic acids, namely DNA and RNA, determines their functions and involvement in vital cell processes including transcription, replication, DNA repair and translation. However, experimental probing of nucleic acids compaction is not straightforward. In this study, we suggest an approach for this probing based on the low-frequency Raman spectroscopy. Specifically, we show theoretically, computationally and experimentally the quantifiable correlation between the low-frequency Raman intensity from nucleic acids, magnitude of thermal fluctuations of atomic positions, and the compaction state of the biomolecules. Noteworthily, we highlight that the LF Raman intensity differs by an order of magnitude for different samples of DNA, and even for the same sample in the course of long-term storage. The feasibility of the approach is further shown by assessment of the DNA compaction in the nuclei of plant cells. We anticipate that the suggested approach will enlighten compaction of nucleic acids and its dynamics during the key processes of the cell life cycle and under various factors, facilitating advancement of molecular biology and medicine.
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