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Proteomic and transcriptomic analyses of Cutibacterium acnes biofilms and planktonic cultures in presence of epinephrineстатья
Дата последнего поиска статьи во внешних источниках: 12 июня 2024 г.
- Авторы: Gannesen AV, Schelkunov MI, Ziganshin RH, Ovcharova MA, Sukhacheva MV, Makarova NE, Mart'yanov SV, Loginova NA, Mosolova AM, Diuvenji EV, Nevolina ED, Plakunov VK
- Журнал: AIMS MICROBIOLOGY
- Том: 10
- Номер: 2
- Год издания: 2024
- Первая страница: 363
- Последняя страница: 390
- DOI: 10.3934/microbiol.2024019
- Аннотация: Transcriptomic and proteomic analysis were performed on 72 h biofilms of the acneic strain Cutibacterium acnes and planktonic cultures in the presence of epinephrine. Epinephrine predominantly downregulated genes associated with various transporter proteins. No correlation was found between proteomic and transcriptomic profiles. In control samples, the expression of 51 proteins differed between planktonic cultures and biofilms. Addition of 5 nM epinephrine reduced this number, and in the presence of 5 µM epinephrine, the difference in proteomic profiles between planktonic cultures and biofilms disappeared. According to the proteomic profiling, epinephrine itself was more effective in the case of C. acnes biofilms and potentially affected the tricarboxylic acid cycle (as well as alpha-ketoglutarate decarboxylase Kgd), biotin synthesis, cell division, and transport of different compounds in C. acnes cells. These findings are consistent with recent research on Micrococcus luteus, suggesting that the effects of epinephrine on actinobacteria may be universal.
- Добавил в систему: Ганнесен Андрей Владиславович