Аннотация:Screening for new antibacterial compounds is an urgent need ofmedicinal chemistry. Understanding new antibiotics mechanism of action is neededfor progression in the drug development pipeline. In the frame of the projectsupported by the Ministry of Science, we developed a reporter system which allows an express, cost-effective and high-throughput screening for simultaneous detectionof antibacterial activity, protein synthesis inhibition and induction of DNA damageSOS response. Automation of the screening process developed in the frame of thisproject allowed to screen up to 17,856 compound chemical library, supplied by theindustrial partner of the project, Research Institute of Chemical Diversity. Amongthe tested compounds, DNA damaging agents appeared almost sixfold morefrequently than those that inhibited protein synthesis. Several new families ofantibacterial compounds were found among the tested set.
