Advances of high-throughput sequencing for unraveling biotechnological potential of microalgal-bacterial communities

Zaytsev, P.A.; Rodin, V.А.; Zaytseva, A.A.; Zvereva, M.I.; Solovchenko, A.E.

Авторы: Zaytsev Petr A., Rodin Vladimir А., Zaytseva Anna A., Zvereva Maria I., Solovchenko Alexei E.
Журнал: Journal of Applied Phycology
Год издания: 2024
Издательство: Springer Nature
Местоположение издательства: Switzerland
DOI: 10.1007/s10811-024-03267-1
Аннотация: Although established biotechnological applications of microalgae e.g., the production of high-valuemetabolites is based on axenic cultures, exploitation of the mutualistic consortia of microalgae andbacteria quickly comes to foreground, especially in bioremediation and wastewater treatment. This trendshifts the focus from genomic research of certain microalgal species to metagenomic studies ofinteractions between microalgae and bacteria in natural communities and in artificial consortia.Dissection of the genetic determinants of the robustness and productivity of the consortia become a hotresearch direction, too. Admirable contribution to this topic had been made by high-throughputsequencing (HTS), while recent breakthrough in this field was entailed by the advent and rapiddevelopment of the 3rd generation nanopore sequencing which becomes increasingly accurate whileproviding unprecedented sequencing performance. Recent progress of the Oxford NanoporeTechnologies (ONT) enabled both classical metagenomic analysis of microalgal-bacterial communitiesbased on whole metagenome sequencing as well as taxonomic and genetic profiling based on theamplicon sequencing. The parallel emergence of novel bioinformatic algorithms for processing themetagenomic datasets opened new opportunities for the analysis of structure and physiology ofmicroalgal-bacterial communities. From the practical perspective, the new HTS techniques became atime- and labor-savers in discovery of new microalgae with a high potential for the accumulation ofvaluable metabolites, biodegradation of hazardous micropollutants, and biosequestration of nutrientsfrom waste streams. Search for prokaryotic species boosting the biotechnological potential of eukaryoticmicroalgae via mutualistic interactions with them is another important goal. The insights from the bothshort-read and long-read metagenomics will form a solid foundation for the rational design ofmicroalgal-bacterial consortia for biotechnology. In this review, we briefly outline the benefits of thelong-read sequencing for structural and functional investigation of algal-bacterial consortia andsummarize recent reports on using this approach for achieving the biotechnology-related goals.
Добавил в систему: Соловченко Алексей Евгеньевич

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Advances of high-throughput sequencing for unraveling biotechnological potential of microalgal-bacterial communitiesстатья

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