Streptomyces phaeochromogenes  BV-204, K-1115А Anthraquinone- Producing Strain: A New Protein  Biosynthesis Inhibitor

Belik, A.R.; Zakalyukina, Y.V.; Alferova, V.A.; Buyuklyan, Y.A.; Osterman, I.A.; Biryukov, M.V.

Авторы: Belik A.R., Zakalyukina Yu V., Alferova V.A., Buyuklyan Y.A., Osterman I.A., Biryukov M.V.
Журнал: Acta Naturae (англоязычная версия)
Том: 16
Номер: 1
Год издания: 2024
Издательство: Park Media Ltd
Местоположение издательства: Moscow, Russia (Federation)
Первая страница: 14
Последняя страница: 23
Оригинальные версии: Streptomyces phaeochromogenes БВ-204 – штамм-продуцент антрахинона К-1115А, нового ингибитора биосинтеза белка
DOI: 10.32607/actanaturae.27315
Аннотация: In the search for new antibiotics, it is a common occurrence that already known molecules are“rediscovered” while new promising ones remain unnoticed. A possible solution to this problem may be the so-called “target-oriented” search, using special reporter microorganisms that combine increased antibiotic sensitivity with the ability to identify a molecule’s damaging effect. The use of such test organisms makes it possible to discover new promising properties even in known metabolites. In this study, we used a high-throughput screening method based on the pDualrep2 dual reporter system, which combines high sensitivity through the use of modified strains of test organisms and makes it possible to easily and accurately identify the interaction mechanisms of a substance and a bacterial cell at the initial stages of screening.This reporter system is unknown in Russia and is significantly superior to its global analogues. In the system, translation inhibition induces the expression of the fluorescent protein Katushka2s, while DNA damage is induced by TurboRFP. Using pDualrep2, we have isolated and described BV-204, an S. phaeochromogenes strain producing K-1115A, the biologically active substance that we have previously described. In our study,K-1115A for the first time has demonstrated antibiotic activity and an ability to inhibit bacterial translation,which was confirmed in vitro in a cell-free translation system for FLuc mRNA. K-1115A’s antibacterial activity was tested and confirmed for S. aureus (MRSA) and B. subtilis, its cytotoxicity measured against that for the HEK293 cell line. Its therapeutic index comprised 2 and 8, respectively. The obtained results open up prospects for further study of K-1115A; so, this can be regarded as the basis for the production of semi-synthetic derivatives with improved therapeutic properties to be manufactured in dosage forms.KEYWORDS actinomycetes, K-1115A, antibiotics, reporter system pDualrep2, inhibition of protein biosynthesis,in vitro translation, citizen science.
Добавил в систему: Бирюков Михаил Владимирович

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