Аннотация:Wheat straw is a potential feedstock for sugar production. Ozonation was tested as a pretreatment method for lignin destruction, enhancing the cellulose accessibility to enzymatic digestion. Effect of ozone consumed on wheat straw delignification was investigated. Visual analysis of the lignin removal from the cell walls during ozonation was carried out using scanning electron microscopy. Relationship between the degree of substrate delignification and its reactivity in enzymatic hydrolyses into sugars was elucidated.