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Интеллектуальная Система Тематического Исследования НАукометрических данных |
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Phylogeny and structural modeling of the transcription factor CsqR (YihW) from Escherichia coliстатья
- Авторы: Rybina Anna A., Glushak Roman A., Bessonova Tatiana A., Dakhnovets Artemiy I., Rudenko Alexander Yu, Ozhiganov Ratislav M., Kaznadzey Anna D., Tutukina Maria N., Gelfand Mikhail S.
- Журнал: Scientific reports
- Том: 14
- Год издания: 2024
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- DOI: 10.1038/s41598-024-58492-y
- Аннотация: CsqR (YihW) is a local transcription factor that controls expression of yih genes involved in degradation of sulfoquinovose in Escherichia coli. We recently showed that expression of the respective gene cassette might be regulated by lactose. Here, we explore the phylogenetic and functional traits of CsqR. Phylogenetic analysis revealed that CsqR had a conserved Met25. Western blot demonstrated that CsqR was synthesized in the bacterial cell as two protein forms, 28.5 (CsqR-l) and 26 kDa (CsqR-s), the latter corresponding to start of translation at Met25. CsqR-s was dramatically activated during growth with sulfoquinovose as a sole carbon source, and displaced CsqR-l in the stationary phase during growth on rich medium. Molecular dynamic simulations revealed two possible states of the CsqR-s structure, with the interdomain linker being represented by either a disordered loop or an ɑ-helix. This helix allowed the hinge-like motion of the N-terminal domain resulting in a switch of CsqR-s between two conformational states, “open” and “compact”. We then modeled the interaction of both CsqR forms with putative effectors sulfoquinovose, sulforhamnose, sulfoquinovosyl glycerol, and lactose, and revealed that they all preferred the same pocket in CsqR-l, while in CsqR-s there were two possible options dependent on the linker structure.
- Добавил в систему: Руденко Александр Юрьевич