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Up- and Down-Regulation of Enzyme Activity in Aggregates with Gold-Covered Magnetic Nanoparticles Triggered by Low-Frequency Magnetic Fieldстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2024 г.
- Авторы: Veselov Maxim M., Efremova Maria V., Prusov Andrey N., Klyachko Natalia L.
- Журнал: Nanomaterials
- Том: 14
- Номер: 5
- Год издания: 2024
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 1
- Последняя страница: 14
- Номер статьи: 411
- DOI: 10.3390/nano14050411
- Аннотация: The modern global trend toward sustainable processes that meet the requirements of “green chemistry” provides new opportunities for the broad application of highly active, selective, and specific enzymatic reactions. However, the effective application of enzymes in industrial processes requires the development of systems for the remote regulation of their activity triggered by external physical stimuli, one of which is a low-frequency magnetic field (LFMF). Magnetic nanoparticles (MNPs) transform the energy of an LFMF into mechanical forces and deformations applied to enzyme molecules on the surfaces of MNPs. Here, we demonstrate the up- and down-regulation of two biotechnologically important enzymes, yeast alcohol dehydrogenase (YADH) and soybean formate dehydrogenase (FDH), in aggregates with gold-covered magnetic nanoparticles (GCMNPs) triggered by an LFMF. Two types of aggregates, “dimeric” (with the enzyme attached to several GCMNPs simultaneously), with YADH or FDH, and “monomeric” (the enzyme attached to only one GCMNP), with FDH, were synthesized. Depending on the aggregate type (“dimeric” or “monomeric”), LFMF treatment led to a decrease (down-regulation) or an increase (up-regulation) in enzyme activity. For “dimeric” aggregates, we observed 67 ± 9% and 47 ± 7% decreases in enzyme activity under LFMF exposure for YADH and FDH, respectively. Moreover, in the case of YADH, varying the enzyme or the cross-linking agent concentration led to different magnitudes of the LFMF effect, which was more significant at lower enzyme and higher cross-linking agent concentrations. Different responses to LFMF exposure depending on cofactor presence were also demonstrated. This effect might result from a varying cofactor binding efficiency to enzymes. For the “monomeric” aggregates with FDH, the LFMF treatment caused a significant increase in enzyme activity; the magnitude of this effect depended on the cofactor type: we observed up to 40% enzyme up-regulation in the case of NADP+, while almost no effect was observed in the case of NAD+.
- Добавил в систему: Веселов Максим Михайлович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | nanomaterials-14-00411-with-cover.pdf | 784,2 КБ | 24 февраля 2024 [nlklyachko] | |
| 2. | Supplementary Information | nanomaterials-14-00411-s001.zip | 1,0 МБ | 24 февраля 2024 [nlklyachko] |