HPLC-MS Analysis of Ginsenosides in Morphogenic Cell Cultures of Ginseng (Panax ginseng C.A. Meyer)

Gorpenchenko, T.Y.; Grigorchuk, V.P.; Makhankov, V.V.; Kochkin, D.V.; Vologzhanina, Y.V.; Khandy, M.T.; Chernoded, G.K.; Zhuravlev, Y.N.

Авторы: Gorpenchenko T.Yu, Grigorchuk V.P., Makhankov V.V., Kochkin D.V., Vologzhanina Yu V., Khandy M.T., Chernoded G.K., Zhuravlev Yu N.
Журнал: Russian Journal of Plant Physiology
Том: 70
Номер: 7
Год издания: 2023
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Номер статьи: 172
DOI: 10.1134/s1021443723603087
Аннотация: The composition of triterpene glycosides in different in vitro tissues of cell lines (PgR–rhizogenic and PgG–hemogenic) and roots of ginseng plants (Panax ginseng C.A. Meyer), grown on plantations in Primorsky Krai (Russian Federation) was determined using the HPLC-MS method. More than 60 triterpene glycosides have been detected and characterized. The maximum content of ginsenosides was identified in the leaves of the hemogenic line. The total amount of ginsenosides in the PgG line embryonic tissue was lower compared to all other samples studied mainly due to the amount of protopanaxadiols. In all parts of the PgR line, the content of protopanaxatriol Re was two times higher than in the root of the intact plant. A high content of protopanaxatriols and oleanolic ginsenosides was found in the basal parts of PgR and PgG calli. Thus, morphogenic cell lines not only repeated the pattern of qualitative ginsenosides composition in comparison with individual parts of native roots and leaves but also demonstrated an increase in their diversity and quantity.
Добавил в систему: Кочкин Дмитрий Владимирович

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HPLC-MS Analysis of Ginsenosides in Morphogenic Cell Cultures of Ginseng (Panax ginseng C.A. Meyer)статья

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