Adhesion of Escherichia coli and Lactobacillus fermentum to Films and Electrospun Fibrous Scaffolds from Composites of Poly(3-hydroxybutyrate) with Magnetic Nanoparticles in a Low-Frequency Magnetic Field

Voinova, V.V.; Zhuikov, V.A.; Zhuikova, Y.V.; Sorokina, A.A.; Makhina, T.K.; Bonartseva, G.A.; Parshina, E.Y.; Хоссаин, М.А.; Shaitan, K.V.; Pryadko, A.S.; Chernozem, R.V.; Mukhortova, Y.R.; Shlapakova, L.E.; Surmenev, R.A.; Surmeneva, M.A.; Bonartsev, A.P.

Авторы: Voinova Vera V., Zhuikov Vsevolod A., Zhuikova Yulia V., Sorokina Anastasia A., Makhina Tatiana K., Bonartseva Garina A., Parshina Evgeniia Yu, Хоссаин Мухаммад Асиф, Shaitan Konstantin V., Pryadko Artyom S., Chernozem Roman V., Mukhortova Yulia R., Shlapakova Lada E., Surmenev Roman A., Surmeneva Maria A., Bonartsev Anton P.
Журнал: International Journal of Molecular Sciences
Том: 25
Номер: 1
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 208
Последняя страница: 208
DOI: 10.3390/ijms25010208
Аннотация: Abstract: The ability of materials to adhere bacteria on their surface is one of the most importantaspects of their development and application in bioengineering. In this work, the effect of theproperties of films and electrospun scaffolds made of composite materials based on biosyntheticpoly(3-hydroxybutyrate) (PHB) with the addition of magnetite nanoparticles (MNP) and their com-plex with graphene oxide (MNP/GO) on the adhesion of E. coli and L. fermentum under the influenceof a low-frequency magnetic field and without it was investigated. The physicochemical properties(crystallinity; surface hydrophilicity) of the materials were investigated by X-ray structural analysis,differential scanning calorimetry and “drop deposition” methods, and their surface topography wasstudied by scanning electron and atomic force microscopy. Crystal violet staining made it possible toreveal differences in the surface charge value and to study the adhesion of bacteria to it. It was shownthat the differences in physicochemical properties of materials and the manifestation of magnetoactiveproperties of materials have a multidirectional effect on the adhesion of model microorganisms. Com-pared to pure PHB, the adhesion of E. coli to PHB-MNP/GO, and for L. fermentum to both compositematerials, was higher. In the magnetic field, the adhesion of E. coli increased markedly comparedto PHB-MNP/GO, whereas the effect on the adhesion of L. fermentum was reversed and was onlyevident in samples with PHB-MNP. Thus, the resultant factors enhancing and impairing the substratebinding of Gram-negative E. coli and Gram-positive L. fermentum turned out to be multidirectional, asthey probably have different sensitivity to them. The results obtained will allow for the developmentof materials with externally controlled adhesion of bacteria to them for biotechnology and medicine.
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Adhesion of Escherichia coli and Lactobacillus fermentum to Films and Electrospun Fibrous Scaffolds from Composites of Poly(3-hydroxybutyrate) with Magnetic Nanoparticles in a Low-Frequency Magnetic Fieldстатья Исследовательская статья

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